Eugenol is a major phenolic component with diverse biological activities.However,its difficult to formulate into an aqueous solution due to the poor water solubility,and therefore limit the application.In present study,eugenol nanoliposomes(EN)were prepared by combining ethanol injection method with dynamic high pressure microfluidization(DHPM)method.Good physicochemical characterizations of EN were obtained.The successful encapsulation of eugenol in nanoliposomes was confirmed by Fourier Transform Infrared Spectroscopy(FTIR).A good storage stability of EN was confirmed by its low variation on average diameter and encapsulation efficiency after 8 weeks storage.No oil drops in EN were found after 8 weeks storage at 4 ℃ and room temperature,which suggested that poor water solubility of eugenol was overcome by nanoliposomes encapsulation.Compared with eugenol solution(ES),a relatively good sustained release property was observed in EN.The antibacterial activity of EN against four common food pathogenic bacteria(Staphylococcus aureus,Escherichia coli,Salmonella typhimurium,and Listeria monocytogenes)in both Luria Broth and milk media was evaluated.