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A mixed culture of extremely acidophilic bacteria was conducted for pyrite bioleaching treatment.Community shift,ferrous oxidizing activity and sulfur activity were investigated during bioleaching process.For better understanding role of active bacteria in bioleaching process,both total RNA and DNA were extracted.Dynamic of microbial community was investigated by methods of 16S rRNA clone library,high-throughput sequencing and real-time quantitative polymerase chain reaction.