【摘 要】
：
目的 探讨人参皂苷(Rb)与I-DOPA合用能否在控制帕金森病症状的同时具有神经保护作用.方法 用鱼藤酮诱导大鼠帕金森模型,每天早晚各注射一次,直到出现PD症状为止；药物处理组包括Rb组(40 mg· kg-1· d-1),I-DOPA组(46 mg·kg-1 ·d-1)和Rb+I-DOPA组,在每次给鱼藤酮前1h灌胃给药,模型组给予等量的生理盐水.同时设正常对照组.从造模开始的整个实验过程中,观
【机 构】
：
安徽中医药大学药学院,安徽合肥230038 浙江大学药学院,浙江杭州310011
【出 处】
：
第十六届全国神经精神药理学学术会议
论文部分内容阅读
目的 探讨人参皂苷(Rb)与I-DOPA合用能否在控制帕金森病症状的同时具有神经保护作用.方法 用鱼藤酮诱导大鼠帕金森模型,每天早晚各注射一次,直到出现PD症状为止；药物处理组包括Rb组(40 mg· kg-1· d-1),I-DOPA组(46 mg·kg-1 ·d-1)和Rb+I-DOPA组,在每次给鱼藤酮前1h灌胃给药,模型组给予等量的生理盐水.同时设正常对照组.从造模开始的整个实验过程中,观测各组动物一般活动能力和肢体运动及平衡协调能力.于最后一次行为实验结束后,用多聚甲醛心脏灌注固定脑组织.用免疫组织化学的方法检测TH阳性染色以测定黑质致密部多巴胺胞体和纹状体多巴胺神经纤维末端的损伤情况,并统计各组大鼠损伤发生率及损伤体积(mm3).结果 与正常组比较,鱼藤酮模型组体重逐渐降低并至显著水平(P＜0.01),Rb或I-DOPA单独给药对鱼藤酮诱导的体重降低无影响,而Rb+I-DOPA可显著对抗体重降低.与正常组比较,模型组的抓力和平衡协调能力显著降低(P＜0.01),与模型组比较,Rb+I-DOPA组可显著对抗抓力与平衡协调能力的降低(P＜0.01,P＜0.05),而Rb或I-DOPA单独给药仅有部分改善作用.脑组织免疫组化分析结果与运动行为结果高度一致.60％的模型组动物纹状体出现显著的TH丢失,指示多巴胺神经纤维末端的损伤,损伤体积为1.63±0.47(n=10)；Rb组和I-DOPA组分别为12.5％(损伤体积为0.27±0.17,n=10)和30％(损伤体积为1.04±0.53,n=10)；而Rb+I-DOPA组无任何动物出现损伤情况.结论 Rb对鱼藤酮诱导的大鼠帕金森病有一定的行为改善和神经保护作用,与I-DOPA合用时效果更为显著特别是可有效保护多巴胺能神经,显示了Rb尤其是与I-DOPA合用在治疗帕金森病的应用前景.
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