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In order to investigate whether or not nanoparticles have an effect of neutrophil inflammation on the lung, we examined the expression of cytokine-induced neutrophil chemoattractants (CINCs) family in rat lung by intratracheal instillation study of nickel oxide nanoparticles agglomerates with the micron-level.The count median diameter of nickel oxide nanoparticle agglomerates suspended in saline was 1.34 m (8.41nm of weighted average surface primary diameter).