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Mulberry,a member of the genus Morus ot the family Moraceae.was a Chinese traditional herbal medicine.Polysaccharides from mulberry leaves have significant antihyperglycemic activity and lower blood lipid.In this paper,the conditions of microwave-assisted extraction (MAE) of polysaccharides from mulberry leaves were optimized based on Response Surface Methodology (RSM).Experimental factors and their levels were determined by one-factor tests.Subsequently,the Box-Benhnken experimental design with 3 factors and 3 levels was performed,and the factors influencing the extracted technology were estimated by means of regression analysis.