Purpose To assess extracellular volume (ECV) and ventricular deformation on cardiac MRI cine and T1-mapping images in amyloidosis patients without late gadolinium enhancement (LGE). Methods After informed consent, 13 patients (age, 55.0±10.8years; male/female = 5/8; LVEF = 63.76±11.97 %) with clinically diagnosed amyloidosis and Cardiac MRI (3.0T, Magnetom Skyra Siemens, Germany) proved LGE-free were included. 16 healthy control subjects (age, 40.5±11.2 years; male/female = 6/10; LVEF = 72.36±18.77 %) were included. ECV of the LV was measure on the basal, mid and apex short-axis T1-mapping images (acquired by a prototype MOLLI sequence) using cvi42 software (version 5.3, Circle Cardiovascular Imaging, Canada). Ventricular deformation of the LV was measured as the average total peak systolic strain in longitudinal, radial and circumferential direction on cine images using cvi42 software. Mann-Whitney U test was used. Results In amyloidosis patients without LGE, there were significant differences of the LV basal ECV (33.21±3.84% vs. 27.78±5.58%, p = 0.010) and global ECV (31.33±4.13% vs. 27.69±3.64%, p = 0.036) compared with healthy subjects. There was no significant differences of the LV longitudinal, radial or circumferential strain between two groups. Conclusion ECV assessed on cardiac MRI T1-mapping images is promising to identify the early impairment in amyloidosis patients without LGE.