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Screening for peptides with high specificity and high affinity against specific targets,such as cancer markers,has significant application in biomedical field.Traditional on-bead peptide screening techniques are always multi-step,time-consuming,lowefficiency and difficult to realize on-line characterization.Herein a novel microfluidic system has been designed in order to screen target peptides against specific targets from the "one bead one compound" combinatorial libraries.