基于ITS2序列鉴定苗药金铁锁及其混伪品

来源 :第十四届全国中药和天然药物学术研讨会暨第十三届中药全球化联盟大会 | 被引量 : 0次 | 上传用户:oicq35952268
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  目的:本研究利用ITS2序列对苗药金铁锁及其混伪品进行鉴定研究,从而确保药材质量,保障临床用药安全.方法:提取金铁锁药材及其混伪品基因组DNA,PCR扩增并双向测序获得ITS2序列.所有序列经过CodonCode Aligner拼接后,采用MEGA5.1软件进行序列比对,计算种内和种间kimura 2-parameter(K2P)遗传距离,并构建系统发育树.采用相似性搜索法、最近距离法、NJ树法对ITS2序列鉴定能力进行评估.
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