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We investigated the effect of increasing the optical penetration length.inside polydimethylsiloxane (PDMS)-based photobioreactors (PBRs), upon the photosynthetic cell growth of cyanobacteria.A thin layer of Teflon amorphous fluoropolymers (Teflon AF) was applied inside the PDMS-based PBRs to prevent the light loss at the solid-liquid interface.The Teflon AF layer, with a refractive index (NTeflon =1.31) lower than the PDMS (nPDMS =1.442)and higher than the culture medium (nmedium =1.332), constructed the light waveguide in the PBRs via the total internal reflection.Such a combination of refractive indices led to the prevention of light loss at the interface.Schematic descriptions (a, c) and CCD images (b, d) of the light propagation in the regular PDMS-based PBR and Teflon AF-coated PBR are shown below.