Background and purpose: Methamphetamine(MA)is a highly addictive stimulant that affects to neurotoxicity of the brain.Changes of brain metabolites and brain volume in MA abusers have been documented.However,the relationship between the changes of brain metabolites at a selected location and the changes of brain volume at the corresponding locations has never been reported.Therefore,we aim to explore the association between the change of Magnetic Resonance Spectroscopy(MRS)in 4 voxel locations and the change of brain volume at the corresponding locations or region.Methods: Ten MA users(age=24.5±4.7),and 14 healthy control(HC)(age=22.71±3.36)were recruited to this study.Single voxel MRS was acquired in 4 voxel locations including frontal white matter(FWM),frontal gray matter(FGM),posterior gray matter(PGM)and Basal Ganglia(BG)and using point resolved spectroscopy(PRESS)pulse sequence for scanning.For the brain volume,T1-weighted high resolution images were acquired using steady state 3D FFE pulse sequence,and were analyzed by FreeSurfer Version 5.3.Results: Significant reduction of N-acetylaspartate per Glycerophosphocholine(NAA/Cho)at the left FWM and left BG were found in MA compared to the HC groups,p=0.049 and p=0.039 respectively.The volume reduction of the left FWM was paralleled to the significant reduction of NAA/Cho in MA.In addition,the volume reduction of the left BG and FGM were significantly correlated with the reduction of NAA/Cho at the same location,r=0.425; p=0.022 and r=0.327; p=0.048 respectively.Conclusions: The results of MRS in this study agreed well with other previous studies.Additional information from this study were the association between brain volumes and the brain MRS.