Cloning of fatty acid desaturase 2 gene and molecular identification of A and C genome in Brassica s

来源 :第十九届国际十字花科遗传学会及芸薹属2014年年会 | 被引量 : 0次 | 上传用户:VIPT250
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The fatty acid desaturase 2 (fad2 ) gene was proven to be the major locus for high oleic acid ( C18:1 ).Brassica napus is an amphidiploid species originating from a spontaneous hybridization of Brassica rapa and Brassica oleracea.B.napus contains multiple copies in genome for most of the genes,including fad2 genes.We cloned nine fad2 genes from 3 varieties of B.rapa and 3 varieties of B.oleracea,respectively.Alignment of the nine fad2 sequences from B.rapa and B.oleracea detected 6 single nucleotide polymorphic sites,which resulted in 6 amino-acid substitutions.
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