It is well known that the protein, especially the one which has some post-translational modifications, is available to be enriched using some antibody-based techniques.However, only a small portion of proteins have proper antibody available, so the development of high quality affinity approaches are extremely essential and significant to detect proteins that exist in low abundance in ceils.Aptamers are pieces of single-stranded DNA or RNA molecules generally, as it is similar to antibodies can fold into three-dimensional structures with binding sites that are complementary in shape and charge to target antigens, have played more and more important roles nowadays.