Miniature pig,due to its similarities in size,shape,truly omnivorous nature and physiology to humans,is an ideal mammalian model for general metabolic functions and diabetes studies.This study aimed to investigate the molecular mechanism of insulin resistance in Bama miniature pigs induced by selective breeding and high-fat and high-sucrose diet (HFHSD) for six months.We measured and compared the skeletal muscle protein expression and phosphorylation of mTOR and Akt in insulin-resistant Bama miniature pigs.Akt S473 decreased by approximately 47% in the skeletal muscle of insulin-resistant Bama miniature pigs as compared to the control (non-insulin resistant),while muscle mTOR S2448 was comparable between the two groups.These results indicate that 1) the insulin-desensitizing effect of diet ingestion is not due to mTOR phosphorylation and 2) six-month HFHSD in Bama miniature pigs could be a useful model to study early stage of human insulin resistance.