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目的探讨胶体金检测隐球菌荚膜多糖抗原诊断肺隐球菌病的价值。方法于2013—2015年7月采集浙江大学医学院附属第一医院疑似肺隐球菌病患者123例的血液,采用胶体金法检测隐球菌荚膜多糖抗原并评价效果。结果 123例患者中,经病理活检或培养阳性确诊为肺隐球菌病41例,非肺隐球菌病82例。上述两组分别采用胶体金法检测,其中41例肺隐球菌病患者中阳性30例,检测灵敏度为73.17%;82例非隐球菌病患者检测结果均为阴性,检测特异度为100.00%。结论胶体金隐球菌荚膜多糖抗原检测是一种简单快速的肺隐球菌病筛查方法,具有一定的临床应用价值。
Objective To investigate the value of colloidal gold in detection of cryptococcal capsular polysaccharide antigen in the diagnosis of pulmonary cryptococcosis. Methods Blood was collected from 123 patients with suspected pulmonary cryptococcosis in the First Affiliated Hospital of Zhejiang University from July 2013 to July 2015. Cryptococcal capsular polysaccharide antigen was detected by colloidal gold method and the effect was evaluated. Results Among 123 patients, 41 cases of pulmonary cryptococcosis and 82 cases of non-pulmonary cryptococcosis were diagnosed by pathological biopsy or culture positive. The two groups were detected by colloidal gold method, of which 41 cases of pulmonary cryptococcosis in patients with positive 30 cases, the detection sensitivity was 73.17%; 82 cases of non-cryptococcal test results were negative, the detection specificity was 100.00%. Conclusion The detection of capsular polysaccharide antigen of Cryptococcus pallidum is a simple and rapid screening method for pulmonary cryptococcosis and has certain clinical value.