[Background]Recently,numerous studies have found high selenium (Se) influences the pancreas in mammals.However,little is known about the influence of high Se in the pancreatic tissue in avian.[Objective]We attempted to characterize the relationship between dietary Se,the selenoprotein, and the pancreas in chicken.This project will provide certain help for poultry farming and provide a reference for medicine.[Methods] 1-day-old chickens received different quantities of dietary Se (0.15,3.0, or 5.0 mg Se/kg of diet) for 8 weeks.Blood samples were analyzed immediately for glucose content at 2nd,4th,6th, and 8th week.The pancreas tissue was collected and examined for the activity of selenoenzymes (GPX, TrxR), and antioxidant related factors (T-AOC, MDA, GSH) and mRNA expression of the transcriptome of selenoproteins at 4th and 8th week.[Results]In the 5.0 mg Se/kg group,a significant increase (P<0.05)in blood glucose was observed.Dietary excess Se influenced on four antioxidant factors, such as T-AOC, H2O2, MDA,and CAT.From these data,we observed that excess Se induces decreased antioxidant capacity of pancreatic tissue.At super-nutritional Se (3.0 mg/kg),most selenoproteins were up-regulated in chicken pancreatic,but Gpx3 ,Sepp2,and Selu was only down-regulated.[Conclusion] Dietary Se level can influence the pancreatic antioxidant capacity by regulating the activity and transcription of selenoenzymes.These results indicate that excess Se induces pancreatic dysfunction through selenoproteins.Dietary Se status stabilizes pancreatic normal physiology function via regulation of the selenoprotemic transcriptions.We hypothesize that hierarchy of regulated the transcriptions of selenoproteome makes an important roles of renal Se metabolism and transport in avian.