【摘 要】
：
Liver fibrosis is a frequent, life-threatening complication in modern life.Despite major achievements in the understanding of its pathogenesis, the reversible treatment is still limited.Many studies h
【机 构】
：
First Affiliated Hospital, Zhejiang University School of Medicine,Hangzhou,China
【出 处】
：
The 9th Asian Biophysics Association Symposium (ABA2015)(第九届
论文部分内容阅读
Liver fibrosis is a frequent, life-threatening complication in modern life.Despite major achievements in the understanding of its pathogenesis, the reversible treatment is still limited.Many studies have explored activation of hepatic stellate cells (HSCs) as a key event in fibrogenesis.Quiescent HSCs store retinol ester and triacylglycerol (TAG) in cytoplasmic lipid droplets (LDs).Reduction of LDs is a characteristic feature of HSC activation, but the relationship between LDs and HSC activation still remains unclear.We focus on HSCs as a turning point to alleviate and even to reverse hepatic fibrosis.Since we have established a LD study system including isolation, proteomics, and lipidomics, we analyzed the neutral lipid core and the surface proteins of HSC LDs.Because of the insufficiency quantity of primary HSCs, we isolated LDs using LX-2 human immortalized HSCs.Of reporting the 286 LD-associated proteins in the proteome of HSCs, five major proteins, perilipin2 (ADRP), perilipin3 (Tip47), lanosterol synthase, Acyl-CoA synthetase long-chain acyl-CoA synthetase (ACSL), and hydroxysteroid (17-beta)dehydrogenase constituted about half of the total LD proteins.Interestingly, retinol, oleic acid (OA), and palmitic acid (PA) were not only involved the synthesis of different neutral lipids in the LDs but also altered LD surface protein composition, that in turn played roles in HSC activation.Our results highlight the role of LDs in inhibition of HSC activation.These findings provide some hints toward a therapeutic strategy for treatment of hepatic fibrosis.
其他文献
Fundamental and evolutionarily conserved role of autophagy is adaptation to starvation,however, the exact role of autophagy as nutrient recycle system remains unclear in mammals.We have investigated t
Large-conductance Ca2+-and voltage-activated BK channels, composed of poreforming subunits and auxiliary β subunits, play important roles in diverse physiological activities.The β1 is predominately ex
Cancer stemness plays a pivotal role in tumor progression and therapy resistance, representing a great challenge to cancer treatment.With the evolution of cancer stem cell (CSC) model, compelling evid
Rapid and sensitive detection methods are in urgent demand for the screening of extensively used organophosphorus pesticides and highly toxic nerve agents for their neurotoxicity.In this study, we dev
One of the most important recent findings in cancer genomics is the identification of novel driver mutations which often target genes that regulate genome-wide chromatin and DNA methylation marks.Litt
Epithelial cancer-associated MUC1 glycoprotein serves as an attractive and broadly applicable target for cancer immunotherapy, which contains a variable number of tandem repeats (VNTR).The VNTR compri
Trigeminal sensory system is essential for the orofacial system, and the existence of somatic exocytosis in trigeminal ganglion neurons is well known.While we found that in addition to the classic cal
Many drug molecules inhibit the conduction of several families of cation channels by binding to a small cavity just below the selectivity filter of the channel protein.The exact mechanisms governing d
Enrichment and purification of silymarin from Silybum marianum extracts were performed using five types of macroporous resins.Static tests indicated that HPD-600 resin had the best performance.To opti
Protein aggregation is usually mediated by denaturation and hydrophobic interaction under the presence of water molecule.In general, hydrophilic residues of proteins are exposed outside to maximize th