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催化光度法测定微量砷,近十多年来已有报导。Naidu等利用催化锇——硫脲反应测定As（Ⅲ）的方法,虽较简便快速,但条件要求较高,需恒温控制,再现性与灵敏度都不够理想。本文利用正交试验对试剂浓度进行条件选择,采用同时测量试波与空白液的吸光度最大差值进行测定,既可省略一般催化法需绘制A—t曲线试验条件,且能在4—6℃温差范围内进行实验,简化了需温控±0.5℃的麻烦手续,再现性与灵敏度也有所提高。本文通过调节试剂用量,对相同的As（Ⅲ）含量在三种不同的温度范围内绘得三条基本重合的工作曲线,为催化光度法测定砷在不同的温度下进行提供了可能。As（Ⅲ）含量测定范围为0—3.2μg/ml。
Catalytic spectrophotometric determination of trace arsenic has been reported for more than a decade. Naidu and other catalytic osmium - thiourea reaction determination of As (Ⅲ) method, although more simple and quick, but the conditions require a higher temperature control, reproducibility and sensitivity are not ideal. In this paper, the orthogonal test was used to select the reagent concentration, and the maximum difference between the absorbance of the test solution and the blank solution was measured at the same time. This method can omit the A-t curve test conditions for the general catalytic method, Experiments in the temperature range simplify the cumbersome procedure requiring temperature control ± 0.5 ° C, and reproducibility and sensitivity are also improved. This article by adjusting the amount of reagent, the same As (Ⅲ) content in three different temperature ranges painted three basically coincide the working curve, the catalytic spectrophotometric determination of arsenic at different temperatures to provide the possibility. As (Ⅲ) content determination range of 0-3.2μg / ml.