【摘 要】
:
目的 研究LPS激活的小胶质细胞条件培养基对DA能神经元毒性作用.方法 收集LPS激活的不同浓度小胶质细胞培养基,分别作用于多巴胺能神经元MN9D、胆碱能神经元NG108-15和神经前体细胞RMN,利用MTT方法检测各细胞活性;同时,利用实时定量PCR检测α-synuclein mRNA在各时间点的表达水平.结果 活化小胶质细胞条件培养基作用于MN9D、NG108-15、RMN三种细胞,24小时后
【机 构】
:
首都医科大学北京神经科学研究所,北京神经再生修复研究重点实验室,北京100069
【出 处】
:
中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the
论文部分内容阅读
目的 研究LPS激活的小胶质细胞条件培养基对DA能神经元毒性作用.方法 收集LPS激活的不同浓度小胶质细胞培养基,分别作用于多巴胺能神经元MN9D、胆碱能神经元NG108-15和神经前体细胞RMN,利用MTT方法检测各细胞活性;同时,利用实时定量PCR检测α-synuclein mRNA在各时间点的表达水平.结果 活化小胶质细胞条件培养基作用于MN9D、NG108-15、RMN三种细胞,24小时后,三种细胞活性分别降低44.3%、41.7%和39.9%; 48小时后,分别降低35.2%、31.2%和38.3%; 72小时后,分别升高29%、56.2%和3.3%.MN9D中α-synuclein mRNA表达量24 h升高67.7%,48 h升高60%,72h升高186.3%.结论 活化小胶质细胞条件培养基对多巴胺能神经元MN9D、胆碱能神经元NG108-15和神经前体细胞RMN均有毒性作用,对多巴胺能神经元MN9D的毒性作用更加明显.活化的小胶质细胞能提高多巴胺能神经元cα-synuclein mRNA的表达,可能起到保护多巴胺能神经元的作用.
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