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In this paper,a moving neutralization boundary (MNB) electrophoresis is developed as a novel model of visual offline sample stacking for the trace analysis of heavy metal ions (HMIs).In the stacking system,the cathodic-direction motion MNB is designed with 1.95～2.8 mM HCl + 98 mM KCl in phase alfa and 4.0 mM NaOH + 96 mM KCl in phase beta.