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Garlic (Allium sativum L.) is an important agricultural product, and has variety of dietary and medicinal functions.This study investigated the protective properties of garlic essential oil (GEO) and its major organosulfur component (diallyl disulfide, DADS) against the development of nonalcoholic fatty liver disease (NAFLD).C57BL/6J mice were fed a normal diet or high-fat diet (HFD) with/without GEO (25, 50, and 100 mg/kg) or DADS (10 and 20 mg/kg) for 12 weeks.