Ardisia is a group of famous herbs in China,which has been used as medical plants for more than 900 years.However,the species from the genus are so analogous that it is difficult to discriminate them just by morphological characteristics.DNA barcoding is a new technique that uses a shot and standard fragments of DNA sequences to identify species quickly,accurately and objectively.In this context,we tried to screen an applicable sequence to discriminate 24 species of Ardisia.