Product inhibition is ubiquitous in hydrogen production by metabolic functions of anaerobic organisms.1 As our previous research of E.Harbinese B49 has pointed out,acetate has a strong inhibitory effect on hydrogen production with ethanol-type fermentation.2 Thus,we developed an integrated system involving fermentative hydrogen production and product separation,which take electrodialysis with bipolar membrane as separation unit coupling with an anaerobic bioreactor.