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In case of a flood,colonies of Solenopsis invicta Buren form a raft on the water surface.Colony rafting increases the amount of venom dose in S.invicta,which improves its defensiveness.However,whether colony rafting directly influences the aggressive behavior of S.invicta remains uncertain.This study explores how rafting treatment affects the aggressive behavior of S.invicta individuals and colonies.The results indicate there was no significant change in individual aggressiveness.At colony level,aggressiveness was significantly decreased after rafting.The results further provide direct evidence of behavioral effect of rafting on S.invicta.