Chitosan and chitosan derivatives are becoming increasingly important natural polymers because of their unique combination of properties like biodegradability,biocompatibility and bioactivity,in addition to attractive physical and mechanical properties.Here we wish to report the synthesis and metal-complex of thiourea chitosan.Thiourea chitosan was prepared by the reaction of chitosan with ammonium thiocyanate in ethanol.Antimicrobial activities of the complex were evaluated against three species of bacteria.This complex had an effect on study bacteria,whose minimum inhibition concentrate (M1C) values against bacteria were 2 times lower than those of Thiourea-chitosan; the complex had higher antibacterial activity on Staphylococcus aureus than antibacterial activity on E.coli and Pseudomonas aeruginosa.Key words:Chitosan,Thiourea chitosan,metal complex.