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Inthepresentstudy,weusedrepeatedfreeze-thawcyclesandDNasedigestiontoprepareanacellularamnioticmembranewith anintactBMstructure,andcross-linkeditwithsoluble1-ethyl-3-(3-dimethylaminopropyl)carbodiimide(EDC)toimproveits mechanicalstrengthandbiostability.