【摘 要】
：
目的:探讨维生素D长期规律用药对自发性高血压大鼠肾素及肾素受体的影响.结果:自发性高血压大鼠20只随机分成两组,实验组给予维生素D3μ g/kg溶于0.5ml丙二醇中腹腔注射,每周两次,总计12周;对照组以等容积的丙二醇腹腔注射.每周无创测压法监测大鼠血压变化.12周后将大鼠采血处死,血清用酶联免疫法(ELISA)检测25 (OH) D3、钙和肾素的浓度;RT-PCR法检测实验组和对照组肾脏肾素及
【机 构】
：
新疆医科大学第一附属医院高血压科 武警新疆总队医院高血压科
【出 处】
：
第二十四届长城国际心脏病学会议、亚太心脏大会暨国际心血管病预防与康复会议(The 24th Great Wall Int
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目的:探讨维生素D长期规律用药对自发性高血压大鼠肾素及肾素受体的影响.结果:自发性高血压大鼠20只随机分成两组,实验组给予维生素D3μ g/kg溶于0.5ml丙二醇中腹腔注射,每周两次,总计12周;对照组以等容积的丙二醇腹腔注射.每周无创测压法监测大鼠血压变化.12周后将大鼠采血处死,血清用酶联免疫法(ELISA)检测25 (OH) D3、钙和肾素的浓度;RT-PCR法检测实验组和对照组肾脏肾素及肾素受体mRNA的表达水平;免疫组化观察肾脏和心脏的肾素受体的分布部位及分布强度;HE染色观察大鼠病理改变.
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