【摘 要】
：
As a member of pattem-recognition receptors (PRRs), the Toll-like receptor (TLR) and its signaling pathway play pivotal roles in recognizing various pathoge
【机 构】
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The Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Scien
【出 处】
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18th International Pectinid Workshop(第18届国际扇贝研讨会)
论文部分内容阅读
As a member of pattem-recognition receptors (PRRs), the Toll-like receptor (TLR) and its signaling pathway play pivotal roles in recognizing various pathogen-associated molecular patterns (PAMPs), and buildup the front-line against invading pathogens.In the present study, the sequence features and mRNA expression profiles of five key genes involved in TLR signal pathway were characterized, and their functions in the immune responses were also investigated in order to validate the TLR signaling pathway and its potential roles in the immune defense of Zhikong scallop Chlamys farreri.These five genes, including CfTLR, CfMyD88, CfTRAF6, Cflκ B and CfNκB, exhibited significant similarity with their homologues from other model organisms, and contained the typical motifs.A strong interaction between the TIR domain from CfTLR and CfMyD88 protein was revealed via ELISA assays.The mRNA transcripts of these five genes were all up-regulated after LPS stimulation (Fig.1), indicating that they were involved in the immune response against LPS.
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