【摘 要】
：
Transient receptor potential (TRP) channels are polymodal cation channels that provide one of the main pathways for cation influx in excitable and non-excitable cells.Among them, thermo-sensitive TRPV
【机 构】
：
State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Molecular Medicine, Pek
【出 处】
：
The 9th Asian Biophysics Association Symposium (ABA2015)(第九届
论文部分内容阅读
Transient receptor potential (TRP) channels are polymodal cation channels that provide one of the main pathways for cation influx in excitable and non-excitable cells.Among them, thermo-sensitive TRPV1 and TRPV2 channels are molecular sensors for high temperatures important for physiology and diseases.These channels are nonselective cation channels and permeate Ca2+ on the plasma membrane.The Ca2+ influx through these channels may play major roles in physiological functions, such as regulation of Ca2+ homeostasis and transmitter release.Thus, it is important to determine fractional contribution of Ca2+ to the total ion influx, or their fractional Ca2+ current (Pf) by combing patch-clamp (total current) and fura2 (Ca2+ influx) [1, 2].In the present work, by determining Pf of pore-site-mutations systematically, we have studied structure-function of Pf(TRPV1) and Pf(TRPV2) in HEK293 cells.We found, that Pf of TRPV2 is 5 times of TRPV1 (22 ％ vs.5.4％).A single mutation can switch their Pf each other.Finally, physiological impact of Pf(TRP) was examined in native DRG neurons.
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