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For the successful translational research,it is important to have thorough discussions with clinical doctors to understand the medical needs.The requirements in the marker development would be follows:(1)Decision of the target disease,which is known for many patients and difficulty of diagnosis.(2)Diagnostic procedure that is highly sensitive and specific.(3)Superior sensitivity and specificity than currently available procedures.(4)Applicable in companion diagnostics.(5)Fast diagnosis and easy handling for everyone in the clinical site.