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The fisheries of Lake VictoriAare extremely important to the economy of Uganda,largely throughtheir export to the European Union.Records from the National Fisheries Resources Research Institutehave reported decline in fish stock piles from an average of 1.2 million tons between 1999 and 2007 toabout 0.8 million in 2013.The culprits for the decrease in stock piles could be pollutants like PCDD/Fsand dl-PCBs arising from improper disposal of industrial effluent,sewage and hazardous waste.In spiteof the importance of fish to the diet of the local population,as well as it being an important exportcommodity,Asurvey of literature shows no available datAconcerning the profiles of PCDD/Fs anddl-PCBs in the aquatic environment of Lake Victoria.Therefore,to ascertain the levels two commerciallyimportant fish species,Nile perch (Latesniloticus) and Nile tilapiA(Oreochromisniloticus) belonging todifferent trophic levels were collected from the Napoleon gulf and Thurston Bay in Lake Victoria.Polychlorinated dibenzo-p-dioxins (PCDDs),polychlorinated dibenzofurans (PCDFs) and dioxin-likepolychlorinated biphenyls (dl-PCBs) were extracted from the fish muscles and livers using the 13Cisotope dilution method,followed by multiple column chromatography clean-up.