【摘 要】
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Background Nowadays, natural antitumor materials had caught more and more attention.And essential oil was the important active component of natural products.Annonasquamosa fruit played great antineopl
【机 构】
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College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China
【出 处】
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世界中医药学会联合会中药化学专业委员会第五届学术年会暨蒙医药论坛
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Background Nowadays, natural antitumor materials had caught more and more attention.And essential oil was the important active component of natural products.Annonasquamosa fruit played great antineoplastic activities.Butthe pericarps were discarded as waste.In this article, the anti hepatoma activities of essential oil extracted from Annonasquamosa pericarps and the underlying mechanism of action were described.Essential oil of Annonasquamosapercarps (APEO) was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS).The anti-proliferative activity of APEO on SMMC-7721 cells was assessed by MTT assay and the pro-apoptosis and cell cycle arrest activities were analyzed by fluorescence microscope and flow cytometry.Results A total of 59 compounds were identified in APEO from Annonasquamosa pericarps by GC-MS.The major compound in the oil was(-)-spathulenol (32.51%).The APEO demonstrated the potent anti SMMC-7721 hepatoma cells activity with values of IC50 lower than 55 μg/mL.At the same time,cells incubated withAPEOwere found in different degrees of nucleus shrinkage or broken through fluorescent microscope studies.In addition, pro-apoptosis and cell cycle arrest were confirmed byflowcytometry analysis, especially in the high concentration group.Conclusion:Essential oil of wastes(Annonasquamosa pericarps) did have potential to kill 7721 cells.And the possible mechanisms were pro-apoptosis and cell cycle arrest.
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