【摘 要】
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Aim: The potential of red clover extract in the prevention of lipid disorder has attracted increasing attention increasingly in recent years.In this study, we determined whether and how red clover ext
【机 构】
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School of Life Sciences, Longyan University, Longyan 364000, China Fujian Key Laboratory of Preventi
【出 处】
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2012上海中医药与天然药物国际大会
论文部分内容阅读
Aim: The potential of red clover extract in the prevention of lipid disorder has attracted increasing attention increasingly in recent years.In this study, we determined whether and how red clover extract affect the development of murine diet-induced nonalcoholic steatohepatitis.Methods: Nonalcoholic steatohepatitis was induced in C57BL/6 mice by feeding mice with methionine-choline-deficient (MCD) diet.Hematoxylin and eosin staining were used for histological analyses.Real-time PCR was used to analyze the mRNA expression levels.Results: Hepatic steatosis and necroinflammation was observed in MCD diet-fed mice, and this diet-induced steatosis was significantly attenuated whereas liver inflammation was not significantly attenuated by red clover extract treatment.Consistent with the results of H&E staining, MCD diet-induced increase of liver triglycerides and cholesterols levels were significantly reduced by red clover extract treatment.However, with the improvement in hepatic steatosis, mRNA levels of acetyl CoA oxidase, carnitine palmitoyl transferase-1, liver fatty acid-binding protein, three genes regulated by peroxisome proliferator-activated receptor (PPAR) a, were unaffected.Conclusions: Red clover extract alleviated MCD diet-induced hepatic steatosis, but did not ameliorate liver inflammation in C57BL/6 mice, and the improvement in hepatic steatosis was not through activating PPAR a.
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