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Aim Recent study have reported 2Methoxy6acetyl7methyljuglone (MAM) for its potential antimicrobial, neuroprotective and anticancer activity.However, the antiinflammation effects of MAM remains to be elucidated.We investigated the antiinflammation activity of MAM.Methods RAW 264.7 macrophages were exposed to LPS with or without MAM.Inducible nitric oxide synthase (iNOS) expression and signaling molecules activated by LPS were evaluated.Results LPSinduced iNOS expression and nitric oxide (NO) expression was suppressed by MAM.MAM attenuated p38MAPK in cells treated with LPS.In addition, MAM caused an increase in MKP1 expression, which could suppress p38MAPK phosphorylation.Conclusions MAM may activate MKP1, which then dephosphorylates p38MAPK, resulting in iNOS downregulation in LPSstimulated RAW264.7 macrophages.The present study indicate that MAM may possess the potential to alleviate LPSassociated inflammatory disorders.