【摘 要】
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Objective: To detect the heterogeneous subclones in t(8;21)acute myeloid leukemia (AML) by quantitative multigene fluorescence in situ hybridization (QM-FISH), and to determine whether putative ancest
【机 构】
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State Key Laboratory of Experimental Hematology,Institute of Hematology and Blood Diseases Hospital,
【出 处】
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22nd Asia Pacific Cancer Conference(第22届亚太抗癌大会)
论文部分内容阅读
Objective: To detect the heterogeneous subclones in t(8;21)acute myeloid leukemia (AML) by quantitative multigene fluorescence in situ hybridization (QM-FISH), and to determine whether putative ancestral relationships exist among different subclones.Methods: Bacterial artificial chromosome (BAC) clones containing the target genes AML1, ETO, WT1, p27, and c-kit were searched in the UCSC Genome Bioinformatics database.FISH probes were prepared by linking fluorescein-labeled dUTP or dCTP to targeted genes by nick translation.Bone marrow cells obtained from t(8;21) AML patients were dropped to the wet surface of glass slides after hypotonic treatment and fixation.
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