【摘 要】
：
Objective: To detect the heterogeneous subclones in t(8;21)acute myeloid leukemia (AML) by quantitative multigene fluorescence in situ hybridization (QM-FISH), and to determine whether putative ancest
【机 构】
：
State Key Laboratory of Experimental Hematology,Institute of Hematology and Blood Diseases Hospital,
【出 处】
：
22nd Asia Pacific Cancer Conference(第22届亚太抗癌大会)
论文部分内容阅读
Objective: To detect the heterogeneous subclones in t(8;21)acute myeloid leukemia (AML) by quantitative multigene fluorescence in situ hybridization (QM-FISH), and to determine whether putative ancestral relationships exist among different subclones.Methods: Bacterial artificial chromosome (BAC) clones containing the target genes AML1, ETO, WT1, p27, and c-kit were searched in the UCSC Genome Bioinformatics database.FISH probes were prepared by linking fluorescein-labeled dUTP or dCTP to targeted genes by nick translation.Bone marrow cells obtained from t(8;21) AML patients were dropped to the wet surface of glass slides after hypotonic treatment and fixation.
其他文献
目的 探讨护理干预对ICU综合征的影响.方法 选择我科2012年7月至2013年1月,收治ICU大于2d年龄大于60岁120例骨科术后患者为研究对象,随机分为两组实验组60人,对照组60人,实验组给予充分护理干预通过提高患者的认知力、有效护患沟通提供社会信息、满足老年患者合理的要求、心理护理.对照组不采取任何干预,其中患者性别、年龄、性格、疾病史、经济情况、认知能力均无统计学意义.
目的 探讨间歇充气加压泵(IPC)及序贯加压袜(GCS)对食管癌术后患者下肢深静脉血流动力学的影响.方法 选取我科择期食管癌术后的患者45名,随机分为对照组和两个干预组,对照组采用传统护理方法,干预组采用GCS、IPC两种方法,比较三组在股静脉血流峰值、血流平均速度及血流量指标值的差异.
目的 分析护生在ICU实习期间的关怀能力现状,为培养和提高护生关怀能力提供依据.方法 采用护理专业大学生人文关怀能力量表对我院ICU实习的80名护生进行问卷调查,并结合带教老师与患者多角度评价.结果 调查对象关怀能力评分低于常模(均P0.05);实习时间不同,带教老师关怀能力不同对护生关怀能力均存在差异(P＜0.05);护生对重症患者疾病的恐惧影响护生关怀能力.结论 护生对ICU病人整体关怀能力较
Objective: To examine the levels of circulating endothelial progenitor cells (cEPCs) in the peripheral blood ofnon-Hodgkin lymphoma (NHL) patients and their correlation with the tumor stage.Methods: I
Objective: The clinical outcomes of refractory/recurrent myeloid leukemia salvaged using allogeneic hematopoietic stem cell transplantation (HSCT) are typically poor with high relapse rate and transpl
Objective: To use the novel word mutaome to represent the repertoire of somatic gene mutations in a specific tumor tissue,and to establish a panel of mutation profiling protocol for clinical use in B-
Objective: To use the novel word mutaome to represent the repertoire of somatic gene mutations in a specific tumor tissue and to establish a panel of mutation profiling protocol for clinical use in ac
Objective: To perform a clinical evaluation of the efficacy of chemotherapy combined with IMRT in a non-endemic population affected with early-stage NPC.Methods: Between January 2006 and October 2010,
Objective: To determine whether DNA extracted from free edgefingernail specimens from patients who underwent allogeneic hematopoietic stem-cell transplantation (allo-HSCT) could be used for short tand
Objective: The method of archived bone marrow smears by fluorescence in situ hybridization (FISH) for retrospective diagnosis of hematologic malignancy patients withoutchromosome results was establish