H2S激活的近红外长余辉发光探针的构建与活体成像分析研究

来源 :第十一届全国化学生物学学术会议 | 被引量 : 0次 | 上传用户:sunny_cui
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
  长余辉发光探针相比于荧光探针在活体成像中往往具有更高的信噪比和灵敏度,在活体分析中具有重要的意义。[1]然而,长余辉探针在活体上能选择性地递送到靶位点、并快速开启余辉信号仍是一个挑战。
其他文献
Qualitative and quantitative analysis of N-glycans by matrix assisted laser desorption/ionization mass spectrometry(MALDI-MS) is hampered mainly by the low ionization efficiency of analytes and their
Metalloporphynns are active sites in metalloproteins and synthetic catalysts.[1]We recently used iron protoporphyrin Ⅸ(FePPⅨ) as an intercalating probe for long-range electrochemical electron transfer
The three biological thiols(Cys,Hcy and GSH) can be effectively distinguished by fluorescent probes supported by their pKa values of sulfydryl induced nucleophilic reaction activities differences of-S
本文研制了一种高效的含二硫化物DNA分子闸的金纳米笼(AuNCs)控释生物传感器,用于谷胱甘肽(GSH)的超灵敏、高选择性检测。通过静电相互作用将含有S-S键的寡核苷酸组装在AuNCs表面,以抑制AuNCs负载的罗丹明B(RhB)等荧光分子的释放。
细菌双杂交系统(BACTH)广泛应用于蛋白质相互作用研究。通过BACTH系统和纳米流式检测技术的有机结合,通过对报告因子和相互作用蛋白的双荧光同时检测,我们课题组发展了蛋白质相互作用的单细菌水平检测方法,具有快速、灵敏、高通量等优点。
Protein-membrane interactions plays important roles in topology of protein inserted in the membrane,regulation of protein activity,protein aggregation and signal transduction.
近红外二区(NIR-Ⅱ,1000-1700 nm)荧光成像具有穿透深度深、空间分辨率高、信噪比高等优势在生物成像领域引发了巨大的关注。同时,NIR-Ⅱ 荧光造影剂通常还具有近红外吸收,因此,可作为诊疗试剂同时实现NIR-Ⅱ荧光成像和光热治疗。
In situ visualization of proteins of interest at the single cell level is a frontier subject in cell biology and molecular biology.
The CRISPR-Cas system represents an efficient tool for genome editing and gene function analysis.The ability to perturb and edit the genome in a precise and targeted manner is crucial to understand ge
Optical manipulation appears to be a powerful tool for spatiotemporally controlling various cellular functions.