论文部分内容阅读
An efficient method was built successfully for the rapid separation and purification of 4-trans-l0-hydroxy-2-decenoic acid (10-HDA) from royal jelly by ultrasound-assisted extraction (UAE) in combination with high-speed counter-current chromatography (HSCCC).The UAE conditions including percent ethanol,extraction time and solvent ratio were optimized with an orthogonal test.The 10-HDA in the crude extract was separated and purified by HSCCC with a two-phase solvent system composed of petroleum ether-aether-methanol-water (1∶1∶0.5∶1.5,v/v).98.2 mg of 10-HDA was obtained from 1.0 g of crude extract in one-step separation with the purity of 99.1% as determined by HPLC.The chemical structure of 10-HDA was identified by ESI-MS,1H-NMR and 13C-NMR.