【摘 要】
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An efficient biocatalytic synthesis of intermediate of rosuvastatin (IR) has been developed using a green catalytic route,conducted under mild conditions without additional chiral reagents.Enzyme scre
【机 构】
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State Key Laboratory of Food Science and Technology,Jiangnan University,1800 Lihu Road,Wuxi,Jiangsu
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An efficient biocatalytic synthesis of intermediate of rosuvastatin (IR) has been developed using a green catalytic route,conducted under mild conditions without additional chiral reagents.Enzyme screening indicated lipase A to be the most efficient biocatalyst for IR synthesis.Methanol was identified as the best acyl acceptor by molecular docking and molecular dynamics.The optimal conditions for synthesis of IR were as follows:10 g/L catalyst,3:1 methanol:substrate,200 g/L substrate,iso-octane as solvent,orbital shaking at 200 rpm,and an incubation time of 24 h.
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