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In this paper, the measurements of Single-walled carbon nanotubes (SWCNTs)chirality were studies By high resolution transmission electron microscopy (HRTEM).Under the high resolution imaging, the diameter Of the carbon nanotubes was measured.On the other hand, the nano beam electron diffraction was applied to Determine the spiral angle of SWCNTs.Combining with the high resolution imaging technology and electron diffraction, the chirality of SWCNTs dispersed in different surfactant solutions and separated by muiticolumn-gel chromatography were determined.