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@@PCA produced by Pseudomonas sp. M18 was a new plant-growth-promoting rhizobacteria (PGPR), which had a strong suppressive inhibition activity against Fusarium spp. and several other fungal phytopathogens with economic significance in agriculture as well [1]. Currently, PCA as a new PGPR has been gradually used in the field of agriculture. Therefore, it was of great significance to determine PCA residual of soil in the fields of agriculture and environment. Whereas, there has not been a proper analytic method for the determination of PCA in soil at trace level yet.