Chaetomium globosum can inhibit the growth of many plant pathogens, such as Venturia inequalis, Rhizoctonia solani, Pyricularia oryzae and etc.by producing the hydrolases such as glucanase and chitinase, which can destroy the cell wall of the plant pathogens, lead to the death of plant pathogens, to achieve the prevention and treatment results.In this research, GS115 Pichia pastoris was used as the expression system.First, searching through the GenBank to get theβ-1,3-glucanase EST gene sequence and the primers were designed to clone the sequence.Then, the RNA of C.globosum was extracted to get the cDNA.After that,using EcoR Ⅰ and Not Ⅰ to perform the double-enzyme digestion, the 1β-1, 3-glucanase gene and plasmid pPIC9K was successfully linked and combined together by using T4 ligase.Finally, pPIC9K-cgglu was successfully constructed.After that, electrotransformation was performed in order to transform the recombinant pPIC9K-cgglu into P.pastoris.Since the recombinant of GS 115-pPIC9K-cgglu has AOX promoter, it was induced by using methanol.SDS-PAGE was performed to verify the target protein and the size of the expressed protein was around 54kD.In conclusion, the β-1, 3-glucanase was successfully expressed in P.patoris.