Mesenchymal stem cells (MSCs) are stromal cells that can differentiate into tissues such as bone and cartilage, making them ideal candidates for tissue engineering.MSCs can home to sites of injury, suggesting that they possess migratory capacity; however, mechanisms remain unclear.We have defined the cell surface chemokine receptor repertoire of murine and human MSCs from bone marrow, with a view to determining their migratory activity.Human MSCs were isolated from patients undergoing hip replacement, using density gradient centrifugation and adherent cell culture.Murine MSCs were isolated from the bone marrow of Balb/c mice by a similar process.Expression of chemokine receptors was studied using flow cytometry, and functional analysis was performed using chemotaxis assays and a flow-based transendothelial adhesion and migration assay.