There has been growing interest of microfluidic technology for point-of-care diagnosis since it allows efficient and rapid detection of target proteins from small sample without an expert operator.In these reasons, microfluidics has been considered to be suitable for portable point-of-care testing (POCT) in resource-limited environments [1].Most microfluidic technology for protein detection use fluorescent or radioactive labels to quantify protein binding events [2].Measurement of these labels requires relatively huge and expensive equipment.This is still a challenging issue in realizing a microfluidic portable biosensor.