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Objective To explore the role of hypoxia-inducible factor-1α(HIF-1α) to the expression of telomerase reverse transcriptase (TERT) in the pulmonary arterial smooth muscle cells under anoxia.Methods HIF-1α and TERT mRNA in the pulmonary arterial smooth muscle cells were examined by RT-PCR with the presence of HIF-1 α inducers, inhibitor or oligodeoxynucleotides.HIF-1α protein was examined by Western blot analysis.Results Exposure of PASMCs to hypoxia produced a remarkable increase in HIF-1α and TERT mRNA levels.HIF-1α inducers CoCl2 and DFX markedly increased mRNA expression of HIF-1α., and hypoxia-induced upregulation of HIF-1α and TERT was inhibited by FAS, HIF-1 α inhibitor.CBZ-LLL significantly increased TERT mRNA expression,but it had no effect on the mRNA levels of HIF-1α.The decoy of HIF-1α by its specific binding dsODN blocked the CoCl2-induced increase in TERT mRNA levels in these PASMCs.Conclusion TERT expression in PASMCs is transcriptionally regulated via an HIF-1α pathway under hypoxic conditions.