【摘 要】
:
高通量RNA-测序(RNA-seq)技术对深入理解哺乳动物转录组结构和表达特征有着巨大的影响.医学研究发现动物肝与脑组织功能上有密切的相关性.本研究利用深度RNA-seq技术对荣
【机 构】
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西南大学荣昌校区动医系,重庆402460
【出 处】
:
中国”西南地区农业动物种质资源挖掘与创新利用“学术研讨会
论文部分内容阅读
高通量RNA-测序(RNA-seq)技术对深入理解哺乳动物转录组结构和表达特征有着巨大的影响.医学研究发现动物肝与脑组织功能上有密切的相关性.本研究利用深度RNA-seq技术对荣昌猪肝和脑组织mRNA 进行分析,共获得8.6 Gb 的测序数据,其中91%为干净Reads,43百万Reads 来自于脑而44 百万Reads 来自于肝.该研究通过证实在脑和肝中分别表达的5,575和4,600 差异表达基因揭露了肝、脑的组织特异性.差异表达基因的功能分析显示荣昌猪脑高丰度表达基因前十个功能途径与神经密切相关,而肝则与代谢途径密切相关.此外,83 个神经肽基因转录本,69 个神经肽受体基因转录本,10 个前神经肽转化酶基因转录本,12 个胰岛素样生长因子结合蛋白基因转录本和另外的神经肽相关蛋白基因转录本被证实,并展示了这些神经分子在脑和肝中不同的表达模式.本项研究为荣昌猪种质资源的挖掘与创新利用以及以荣昌猪作为医学模型的研究奠定了很好的分子基础.
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