医用石蜡油在荧光PCR检测HBV-DNA定量质量控制中的应用

来源 :中华医学会第八次全国中青年检验医学学术会议 | 被引量 : 0次 | 上传用户：yyk20071999

【摘要】

：

目的探讨无菌医用石蜡油在荧光PCR 检测HBV-DNA 定量质量控制中的应用价值.方法经荧光定量PCR 扩增好的江苏省临检中心的质控物高低值为室内质控及病人的标本分成组,及将病人的提取物分为两份,一份加定量浓度的无菌石蜡油封管与另一份不封管,以荧光定量PCR 技术扩增原理,采用分组测定HBV-DNA 的含量,连续测定6 个月实验,根据CT 值统计每组的SD,CV,P 值的情况.

【作者】

：

裴兵

【机构】

：

江苏省泗洪县人民医院检验科 223900

【出处】

：

中华医学会第八次全国中青年检验医学学术会议

【发表日期】

：

2014年6期

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