【摘 要】
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siRNA targeting the murine VEGFR2 gene was designed and validated for efficient and robust silencing in vitro, and was then delivered by PEI in vivo to investigate the anti-tumor effect on non-small-c
【机 构】
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Department of Pharmaceutical,Zhujiang Hospital,Southern Medical University,Guangzhou 510282,China
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siRNA targeting the murine VEGFR2 gene was designed and validated for efficient and robust silencing in vitro, and was then delivered by PEI in vivo to investigate the anti-tumor effect on non-small-cell lung cancer xenograffs.The following dosage regimens were tested for their tumor inhibitory effect in vivo: VEGFR2 siRNA, EGFR siRNA, VEGFR2 siRNA + EGFR siRNA, cisplatin alone, and VEGFR2 siRNA + EGFR siRNA + cisplatin.Targeted silencing of both VEGFR2 and EGFR expression by siRNA, combined with low dose cisplatin was found to effectively inhibit tumor growth, and extend the survival time of mice bearing the NSCLC xenografts.These results suggest that combination therapy using siRNAs and chemotherapy agents might offer a novel strategy for cancer treatment in the future.
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