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将酵母培养物按照0mg/kg.BW(空白对照组)、50.0mg/kg.BW(试验I组)、100.0mg/kg.BW(试验Ⅱ组)、150.0mg/kg.BW(试验Ⅲ组)、200.0mg/kg.BW(试验Ⅳ组)、250.0mg/kg.BW(试验Ⅴ组),另以添加量为150.0mg/kg.BW的另一种酵母免疫多糖为药物对照组,定量添加在黄颡鱼(Pelteobagrus fulvidraco)饲料中,连续投喂28d后,取其供试鱼血液,测定了其血液中白细胞的吞噬活性、血清溶菌活力和补体活性,采用嗜水气单胞菌(Aeromonas hydrophila)活菌攻毒,比较了试验鱼的非特异性免疫功能及其对致病性细菌感染抵抗力的差异。研究结果表明,试验Ⅱ～Ⅲ组与药物对照组黄颡鱼白细胞吞噬活性和溶菌酶的活性均显著高于对照组(t测验,P<0.05),而试验Ⅰ组、Ⅳ组和Ⅴ组与对照组黄颡鱼血液中白细胞的吞噬活性和溶菌酶活性没有显著差异(t测验,P>0.05),补体C3和C4活性最高的是投喂量为100.0mg/kg.BW和150.0mg/kg.BW的第Ⅱ组与第Ⅲ组,两个组与对照组间存在显著差异(t测验,P<0.05),而第Ⅰ组、第Ⅳ组和第Ⅴ组与对照组之间无显著性差异(t测验,P>0.05)。活菌攻毒的结果表明,给予酵母培养物的第Ⅱ组、第Ⅲ组和第Ⅳ组与药物对照组之间没有显著差异(t测验,P>0.05)。在本试验研究条件下所取得的结果显示,在黄颡鱼饵料中添加酵母培养物的量以150.0～200.0mg/kg.BW左右为宜,超过250.0mg/kg.BW的添加量后,黄颡鱼的非特异性免疫功能及其对致病性细菌感染抵抗力没有出现进一步改善的趋势。
The yeast culture was administered at 0 mg / kg.BW (blank control), 50.0 mg / kg.BW (test group I), 100.0 mg / kg.BW (test group II), 150.0 mg / kg.BW ), 200.0mg / kg.BW (group Ⅳ), 250.0mg / kg.BW (groupⅤ), and another yeast immunopolysaccharide with a dosage of 150.0mg / kg.BW as the drug control group Added to feed of Pelteobagrus fulvidraco, continuously fed for 28 days, the blood of the test fish was taken, the phagocytic activity of leucocyte, the activity of serum bacteriolytic activity and the complement activity were measured, and the concentration of Aeromonas hydrophila Aeromonas hydrophila, and compared the non-specific immune function of test fish and its resistance to pathogenic bacterial infection. The results showed that the phagocytic activity of leucocyte and the activity of lysozyme in experimental group Ⅱ ~ Ⅲ and control group were significantly higher than those in control group (t test, P <0.05), while those in group Ⅰ, Ⅳ and Ⅴ There was no significant difference in leucocyte phagocytic activity and lysozyme activity in the control group (t test, P> 0.05). The highest complement C3 and C4 activities were fed at 100.0 mg / kg.BW and 150.0 mg / kg .BW Group II and Group III, there was a significant difference between the two groups and the control group (t test, P <0.05), while Group I, Group IV and Group V were not significantly different from the control group Differences (t test, P> 0.05). The results of viable cell challenge showed no significant differences (Group T, P> 0.05) between Group II, Group III, and Group IV administered yeast cultures and the drug control group. The results obtained under the experimental conditions showed that the amount of yeast culture added to the diet of yellow catfish was preferably about 150.0-200.0 mg / kg.BW, and when the amount of 250.0 mg / kg.BW was added, yellow Nonspecific immune function of mullet and its resistance to pathogenic bacterial infection showed no further improvement.