Exploration of human chromosomes by biomass spectrometry based proteomics with trans-omics

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In order to explore the chromosome proteome in a detailed way, more elaborate experiments were designed to perform a biomass spectrometry based proteome using the same hepatoma cell lines (Hep3B,MHCC97H and HCCLM3).In addition, transcriptome and translatome were considered as background expression, upon which several issues associated with chromosome-centric proteome were explored in a quantitative way.In total, we obtained a large dataset with 9,064 identified proteins, and 12,740 proteins with contribution from previous studies which is 62.9% coverage against the Swiss-Prot database.
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