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Metabolism is central to cell biology and influences nearly all cellular processes, whereasmetabolic enzymes play important roles in various metabolic pathways.Pyruvate kinase(PK) catalyses the final step of glycolysis, transfers the high-energy phosphate group fromphosphoenolpyruvate (PEP) to ADP to produce ATP and pyruvate, and acts as a centralnode in the glycolysis pathway.