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Polyaniline(PANI) doped by natural acid(citric acid, salicylic acid or rosin acid)solutions was synthesized using aniline as monomer and ammonium peroxydisulfate ((NH4)2S2O8) as oxidant.The as synthesized PANI was characterized by using different analytical techniques such as field emission scanning electron microscopy(FE-SEM), Fourier transform Infrared spectroscopy (FT-IR), and X-ray diffraction (XRD).The antibacterial activities of the PANI against Gram positive bacteria(Bacillus megatherium, Bacillus subtilis, Bacillus cereus and Staphylo-coccus aureus) and Gram negative bacteria(Bacillus coli) were investigated.It was shown that PANI doped by different acids exhibited different antibacterial activities against different bacteria.PANI doped by citric acid possesses the better antibacterial activity against the selected species of Gram positive bacteria and Gram negative bacteria than PANI doped by rosin acid.PANI doped by salicylic acid exhibits good antibacterial activities against Gram positive bacteria, but it has little antibacterial activity against the Gram negative bacteria.